动物造模 z-bio 2021-09-15 220

　　OBJECTIVE: To discuss the protective effect of cysteinyl leukotriene receptor antagonists (Plukast, HAMI 3379) on global cerebral ischemia-reperfusion injury in Mongolian gerbils and its mechanism.

　　Methods: The bilateral common carotid arteries were ligated for 10 minutes and reperfusion for 24 hours to establish a model of global cerebral ischemia-reperfusion injury in gerbils. They were randomly divided into sham operation group, model group, Plukast group, and HAMI 3379 group. Group of 20 rats were given intraperitoneal injection 3 days before operation, once a day, 30 min before operation. Observe the neuropathy and function of 24h after reperfusion; observe the neurons in the cortex and hippocampus by Nissl staining method; detect the expression of autophagy-related proteins beclin-1 and LC3 in the cortex and hippocampus by immunoblotting; observe the autophagosomes in the hippocampus by electron microscope . Results Compared with the model group, Plukast and HAMI 3379 groups can improve the scores of neurological disorders, reduce nerve function damage, reduce the damage and loss of neurons in the cortex and hippocampus, and reduce the expression of autophagy-related proteins beclin-1 and LC3 and the hippocampus The number of autophagosomes in the region.

　　Conclusion: Cysteinyl leukotriene receptor antagonists can reduce the cerebral ischemia-reperfusion injury of Mongolian gerbils by down-regulating autophagy in the cerebral cortex and hippocampus.