关节炎模型 z-bo 2020-08-10 554

　　(1) Replication method Dissolve egg protein in physiological saline to make a 20g/L solution, and then mix it with the same amount of Freund's adjuvant. The animals were injected subcutaneously on the back once a week for 3 consecutive weeks to sensitize, and 5 mg of dissolved egg protein was injected into the joint 2 weeks after the last injection.

　　(2) Features of the model Within 24 hours after injection, the joints of the injection site of the model animals showed acute inflammation such as redness, swelling, heat and pain, and the incidence rate reached 100%. The pathological changes of ovalbumin-induced arthritis model include hyperplasia, pannus formation, cartilage and bone destruction. The first stage is the swelling of the joint 24 hours after the injection of antigen into the joint. The joint diameter can be increased by 32%, and the pathological manifestations are acute synovitis and large amounts of exudate. Subsequently, the joint swelling was reduced, but it was still larger than normal joints. The second stage is 1 to 4 weeks, the joint synovium proliferates significantly and pannus forms. Synovial cells increased from 1 to 3 layers to 5 to 10 layers, mainly monocytes and macrophages, followed by lymphocytes, and CD4+ lymphocytes were more common. Early cartilage destruction may occur in some animals at this stage. In the third stage, after 4 weeks, irreversible articular cartilage and bone destruction appeared, with chondrocyte necrosis, cartilage fibrosis, subchondral new bone formation, and finally bone deformation. Until the 6-month observation period, chronic inflammation still exists.

　　(3) Comparative medicine. The pathogenesis of ovalbumin-induced arthritis model is mainly due to the persistence of antigens in the joints, which stimulate synovial cells to secrete antibodies and form antigen-antibody-C3 complexes, which make synovitis persist and synovial hyperplasia , The formation of pannus. The radioisotope-labeled antigen was injected into the rabbit joints. It was observed that the antigen was distributed in the avascular and fiber-rich tissues, excreted slowly, and persisted for 6 months. The ovalbumin-induced arthritis model can be replicated in rabbits, sheep and other animals. Since inbred animals are not required, inflammation is easy to replicate and the joints are relatively large. It is more suitable for the treatment of rheumatoid arthritis. Dynamic therapy, non-invasive treatment, etc.