门静脉高压 z-bio 2021-09-15 557

　　Objective: To study the effect of simple portal hypertension on serum endotoxin and intestinal mucosa in rats?

　　Methods: 16 rats were selected and divided into a blank control group (4 rats) and a model group (3 d group? 7 d group? 10 d group, 4 rats in each group). 3 d to 7 d to 10 d blood and rat jejunum, ileum and colon were collected, and the serum endotoxin content was detected by ELISA method to observe the changes of endotoxin, and the intestinal tissue of each group was collected to stop HE staining and histopathology Observation and analysis?

　　Results: The rats in the model group successfully constructed a partial portal vein ligation model? The results of serum endotoxin at three different time points were not significantly different from those in the normal control group. After 3 days of modeling, no major intestinal segment had occurred. Injury; each intestine segment showed partial destruction and swelling compared to the local intestinal villi of the mucosal layer after 7 days of modeling, and the connection between epithelial cells and the lamina propria showed a loose state; 10 days after modeling was compared with 3 days and 7 days The intestinal mucosal layer and lamina propria showed obvious damage?

　　Conclusion: In the case of normal liver function, portal hypertension can cause intestinal mucosal damage in a short time, but the endotoxin produced by the intestine will not exceed the liver's ability to handle it and show an increase in serum endotoxin?