动物造模,缺氧缺血性脑病 z-bio 2021-09-15 358

　　Objective: To create a hypoxic-ischemic encephalopathy (hyPoxic?ischemic encePhaloPathy, HIE) model in reborn rats, to study the brain maintenance effect of recombinant human erythropoietin (EPO) and the diversity of HIE intestinal flora in reborn rats Changes, provide experimental evidence for the clinical application of EPO in the treatment of neonatal hypoxic-ischemic encephalopathy?

　　Methods: Seven-day-old SD rats were selected to make HIE models, and they were randomly divided into HIE model group, EPO experimental group, and control group. 16s rRNA sequencing method to observe the changes of intestinal flora?

　　Results: The expression level of nestin in each group of rats at the same time point was significantly different (P<0.05), the control group was the lowest, the EPO experimental group was the highest, and the HIE model group followed by the Shannon Wiener index (Shannon Wiener index of the HIE model group). index) Compared with the control group?

　　Conclusion: Exogenous administration of EPO can promote the growth of nerve cells in the HIE reborn rat model, and has a certain maintenance effect. At the same time, the diversity of the intestinal flora of rats has been changed?