动物造模 z-bio 2021-09-15 298

　　Objective: To compare the effects of different local intervention temperatures on endoplasmic reticulum stress (ERS) and cell apoptosis in rats with pressure ulcers in order to provide experimental evidence for clinical prevention and treatment of pressure ulcers.

　　Methods: The pressure ulcer model was established by the ischemia-reperfusion method, and 40 SPF adult male SD rats were randomly divided into 4 groups of sham operation group (sham group), model group, heat intervention group and cold intervention group. The end of the experiment was The muscle tissues of the compressed parts of rats in each group were cut on ice, and the pathological changes of skeletal muscle were observed by HE staining; the expression levels of endoplasmic reticulum stress-related proteins GRP78?casPase-12 and CHOP were detected by Western blot; the expression levels of casPase-12 and CHOP were detected by immunofluorescence. 12 and CHOP expression; TUNEL staining to observe cell apoptosis?

　　Results: Compared with the model group, the degree of skeletal muscle cell damage in the heat intervention group increased, and the apoptotic cells increased. The immunofluorescence results showed that the positive expression of casPase? 12 and CHOP increased, and the Western blot results showed that the GRP78? casPase-12 and CHOP increased. The expression was higher than that in the model group (P<0.05); while in the cold intervention group, the damage of skeletal muscle cells was reduced, and the apoptotic cells were reduced. The expression of 12 and CHOP was lower than that of the model group (P <0.05)?

　　Conclusion: Local cold intervention reduces pressure ulcer injury in rats by inhibiting the apoptotic pathway mediated by endoplasmic reticulum stress; local heat intervention aggravates ERS and promotes the onset of apoptosis, adding severe pressure ulcer injury to rats?