动物造模,抑郁症 z-bio 2021-09-15 265

　　Objective: Taking SD rats as the research object, to explore the effect of histamine on the neurons in the hippocampus C1 area and the effect on the behavior of depression rats?

　　Method: Observe the direct effect of histamine on the firing frequency of neurons in the hippocampus C1 area through the method recorded outside the brain slice in vitro? After the method recorded outside the body cell, stimulate the hypothalamus to observe the endogenous histamine. The effect on the firing frequency of hippocampal neurons? After the open field experiment and the Morris water maze experiment, observe the changes in motor desire and spatial memory ability of depression model rats after microinjection of histamine in the hippocampal C1 area?

　　Results: The data from the extracellular records of the isolated brain slices showed that histamine produced a concentration-dependent excitatory effect on hippocampal neurons through H1 receptors; the records outside the somatic cells showed that endogenous histamine exhibited short-term excitement on hippocampal neurons first and then The two-way effect of long-term inhibition? In behavioral experiments, the injection of histamine in the hippocampal C1 area significantly reduced the desire to exercise in the depressed rats in the open field experiment. In the Morris water maze experiment, the spatial memory can be significantly weakened, while the injection of histamine H3 Can the receptor blocker clobenProPit significantly improve the motor desire and spatial memory ability of depression rats?

　　Conclusion: In the cell and overall level, analysis from different perspectives in vitro and in vivo indicated that histamine exhibits a bidirectional effect of first excitement and then inhibition on neurons in the C1 region of the hippocampus. It exhibits a comprehensive inhibitory effect, which can increase the desire for movement in depression rats. Spatial memory is impaired, and the histamine H3 receptor blocker clobenProPit can improve the desire for exercise and spatial memory in depression rats?