动物造模,脑缺血再灌注损伤 z-bio 2021-09-15 806

　　Objective: To discuss the neurotherapeutic effect and mechanism of IL-6/STAT3/miR-21 in pine bark extract on cerebral ischemia-reperfusion injury in mice.

　　Method: 120 KM mice were randomly divided into normal control group, sham operation group, 14 d and 28 d model group, 14 d and 28 d treatment group, 20 mice in each group, and were manufactured by bilateral common carotid artery clipping method Cerebral ischemia-reperfusion injury model; the treatment group was given pine bark extract proanthocyanidin 7 days before death, and the control group and the sham operation group were given the same amount of normal saline. The differences in learning and memory abilities of each group of mice, the histopathological changes in hippocampus, the expression of interleukin 6, STAT3, p-STAT3 protein, and miR-21 were detected.

　　Results: At the same time, the mice in the node treatment group had better learning and memory ability than the model group mice; the Nissl bodies of the treatment group were arranged disorderly, and some of the cells were irregular in shape and the cytoplasm was relatively lightly stained, but it was better than the model group; 28 days The expression level of IL-6 in the model group was lower than that in the 14-day model group. At the same time point, the IL-6 in the hippocampus of the treatment group was significantly lower than that in the model group; the total protein content of STAT3 in the hippocampus was not significantly different among the groups (P>0.05) ), the p-STAT3 content in the treatment group at the same time point was lower than that in the model group; the expression of miR-21 in the hippocampus tissue at the 14th and 28th d treatment group was lower than that in the model group at the same time point, and decreased with time.

　　Conclusion: The proanthocyanidins of pine bark extract can effectively inhibit IL-6, significantly reduce the phosphorylation of STAT3, and ultimately reduce the expression of miR-21, thereby playing a role in alleviating the brain injury of mice with cerebral ischemia and reperfusion.