动物造模 z-bio 2021-09-17 809

　　OBJECTIVE: To observe the effect of sodium metavanadate (NaVO3·2H2O) exposure on the inflammatory response and migration of microglia, and to discuss the related mechanisms of vanadium neurotoxicity.

　　Method: Incubate SD rat microglia in primary culture with sodium metavanadate, immunofluorescence technique to show the changes in microglia morphology and the expression of specific marker Iba1, and western blot technique to detect iNOS, COX-2, ERK and p-ERK protein expression, enzyme-linked immunosorbent assay was used to detect the release of inflammatory factors TNF-α and IL-1β; a scratch migration model was constructed, and immunofluorescence technology recorded the effect of sodium metavanadate on the migration of microglia.

　　Results: After incubating microglia with sodium metavanadate, the morphology of neuromicroglia changed from a resting state to a phagocytic cell-like morphology, and the expression of its specific marker Iba1 was significantly increased; iNOS and COX-2 proteins The expression and the release of TNF-α and IL-1β were significantly higher than those in the control group; sodium metavanadate promoted the migration of microglia.

　　Conclusion: Sodium metavanadate significantly promotes the inflammatory response and migration of microglia.