动物造模,骨关节炎 z-bio 2021-09-17 790

　　OBJECTIVE: To establish a mouse knee osteoarthritis model by knocking out the SIRT1 gene, and to observe the differences in the morphology of cartilage tissue slices by various separate staining or compound staining techniques.

　　Methods: The mouse knee joint tissue samples were divided into 2 groups: SIRT1-/- mouse sham operation group (n=6, group A), SIRT1-/- mouse osteoarthritis model group (n=6, group B) ). The knee joint osteoarthritis model was established by anterior cruciate ligament transection and medial meniscus resection, and HE staining, Safranin O-fast green staining, Safranin O-alcian blue staining, Safranin O staining, fast green staining, and Alli Neoblue staining, 6 kinds of single staining or combined staining methods to observe the morphological changes of knee cartilage tissue.

　　Results: Safranin O-fast green staining and Safranin O-alcian blue staining were used in observing the cell morphology of chondrocytes, the layered structure of cartilage, the display of type II collagen fibers, the changes in tide lines and subchondral bone. The effect is better; Safranin O staining and Alcian blue staining have certain advantages in observing cartilage tissue loss.

　　Conclusion: In observing the osteoarthritis cartilage tissue sections of the knee joint of SIRT1 knockout mice, compared with single staining, composite staining has more obvious advantages in obtaining various information about cartilage structure.