动物造模,黑色素瘤生长 z-bio 2021-09-17 196

　　Objective: To discuss the effect of Mac-1 deletion on the growth of melanoma tumors.

　　Method: Mac-1 knockout (Mac-1-/-) mice are expanded to obtain the required number of mice for the experiment. A control group of C57BL/6J mice and an experimental group of Mac-1-/- mice were established, and B16-F10 cells were injected subcutaneously. The survival rate of mice was counted, tumor volume and weight were measured, and tumor cell proliferation and tumor cell proliferation were detected by immunohistochemical staining. The status of macrophage infiltration.

　　Results: The survival rate of melanoma mice after Mac-1 deletion was significantly different from that of the control group C57BL/6J mice (P<0.001), and the tumor volume and weight were significantly different from those of the control group C57BL/6J mice (P <0.001), there is a significant difference in the number of proliferating cells in the tumor tissue compared with the control group (P<0.01), and the infiltration of macrophages in the tumor tissue is significantly reduced.

　　Conclusion: Mac-1 gene deletion significantly inhibited the growth of melanoma tumors.