动物造模,乳腺癌肿瘤 z-bio 2021-09-17 569

　　Objective: To study the effect of a single intraosseous injection of simvastatin on the vascular structure and function of breast cancer tumors in mice.

　　Method: To construct an orthotopic model of mouse breast cancer and give a single intraosseous injection of simvastatin (50 μg) or a blank vector. α-SMA/CD31 immunofluorescence double staining was used to observe the pericyte/vascular endothelial cell concealment rate of tumor blood vessels; Evan’s blue dye was injected into the tail vein to observe tumor vascular permeability; immunohistochemistry was used to detect the change of HIF-1α expression in the tumor.

　　Results: Compared with the control group, immunofluorescence double staining showed that the pericytosis of the tumor in the simvastatin group was increased (P<0.05); the content of Evans blue in the tumor tissue was significantly decreased (P <0.05), indicating that the permeability of the tumor blood vessels was decreased. Tumor blood vessels tend to mature; immunohistochemistry shows that the expression of HIF-1α in the tumor is reduced, and the hypoxia of breast cancer tumor tissue is improved.

　　Conclusion: A single intraosseous injection of simvastatin can improve the pericyte covering of regenerated blood vessels and promote the normalization of breast cancer tumor blood vessels.