动物造模 z-bio 2021-09-27 233

　　OBJECTIVE: To observe the effect of fibroblast growth factor-2/polylactic acid-polyethylene glycol-polylactic acid/bone morphogenetic protein-2 (FGF-2/PELA/BMP-2) microcapsule scaffold on SD rat periosteum-derived stem cells Bone differentiation.

　　Methods: Extracting SD rat periosteal stem cells (PDSC), discontinuing the certification of flow cytometry surface markers and the induction of osteogenesis, cartilage, and adipogenesis, and discontinuing Alizarin Red, Toluidine Blue, Alcian Blue, and Oil Red O staining and immunofluorescence experiment. Prepare FGF-2/PELA/BMP-2, FGF-2/PELA, PELA/BMP-2, PELA four groups of materials, stop scanning electron microscopy (SEM) to observe the surface of the microcapsules, and create a two-factor controlled release curve through an ELISA experiment. The four groups of material extracts and the third generation of periosteum-derived stem cells were co-cultured. The alkaline phosphatase (AKP) activity test was stopped on 7 and 14 days, and the qRT-PCR osteogenic gene was stopped on 7, 14, and 21 days. Expression detection, observe and compare the osteogenic differentiation ability of each group of PDSC, and stop statistical analysis after data collection.

　　Result: The flow cytometry surface markers show that PDSC expresses the surface markers of mesenchymal stem cells. The three-line induced differentiation staining results show that PDSC has multiple differentiation capabilities such as osteogenic, cartilage, and adipogenesis. The results of AKP activity showed that the FGF-2/PELA/BMP-2 group had the highest AKP activity on the 7th and 14th day after PDSC incubation, and the difference was significant. The qRT-PCR results of the FGF-2/PELA/BMP-2 group indicated that the expression levels of RunX-2 and OCN were higher than those of other groups, and the expression level of RunX-2 reached the peak on the 14th day, and the OCN showed a continuous increase trend.

　　Conclusion: The cytokine activity in the FGF-2/PELA/BMP-2 biomimetic controlled-release microcapsule scaffold is kept, and has a higher ability to promote PDSC osteogenic differentiation than other experimental groups.