动物造模 z-bio 2022-02-01 384

　　Objective: To investigate the effect of alcohol exposure during pregnancy on basic rhythmic respiratory discharge activity (RRDA) in the medulla oblongata slices of offspring of newborn rats.

　　Methods: To prepare the isolated medulla brain slice specimens of neonatal rats containing the medial region of the nucleus retrofacialis (mNRF) and preserve the hypoglossal nerve root, and give the modified Kreb's solution (MKS) perfusion. , Use the adsorption electrode to record the RRDA of the hypoglossal nerve root on the ventral surface of the medullary brain slice, and analyze the change of RRDA. The experiment was divided into 5 groups: control group, 4% alcohol exposure group, 6% alcohol exposure group, 8% alcohol exposure group, and 10% alcohol exposure group to study the effects of alcohol exposure at different concentrations during pregnancy on RRDA.

　　Results: The RRDA in the control group, 4% alcohol exposure group, 6% alcohol exposure group, and 8% alcohol exposure group within 50 minutes of inhalation time course (TI), respiratory frequency (RF), discharge integral amplitude (IA ) There was no statistical difference. The RRDA rhythm of the 10% alcohol exposure group was irregular; compared with the control group, the RRDA of the 4%-10% alcohol exposure group gradually decreased with the increase of alcohol concentration, TI shortened, RF decreased, and IA weakened.

　　Conclusion: Alcohol exposure during pregnancy inhibits the hypoglossal nerve root RRDA in the medulla oblongata of the offspring of newborn rats, which may be the basis for the inhibition of the offspring’s respiratory function by alcohol exposure during pregnancy.