动物造模 z-bio 2021-10-15 290

　　Objective: To explore the role of up-regulation of KA1 subreceptor expression in the death of hippocampal neurons caused by endoplasmic reticulum stress.

　　Method: 70 adult male Kunming mice were randomly divided into kainic acid group (KA group), tunicamycin group (TM) 500 μg/ml group, TM1000 μg/ml group and TM2000 μg/ml group, in the hippocampus KA or different concentrations of TM were injected, and the brains were perfused at different time periods (1, 2, 3, 4, 5, 8, 12 h), and the brains were perfused for Bederson sign scoring. Whole brain sections were stained with FJB and immunohistochemical analysis.

　　Results: At the 3rd, 4th, 5th and 8th hour of the KA group and the 4th and 5th hour of the TM2000 μg/ml group, the Bederson sign score indicated that the central nervous function was obviously damaged, FJB staining showed increased cell death in the hippocampus, and immunohistochemistry showed KA1 and The expression of P-eIF2α in hippocampal neurons was significantly up-regulated (P<0.05).

　　Conclusion: After intra-hippocampal injection of KA or TM, the results show that KA1 is expressed in endoplasmic reticulum stress. In the process of neuronal apoptosis, the extramembrane receptor KA1 may be the first to bear the apoptotic signal and transmit it to the cell to cause endoplasmic reticulum stress. Reticulum function disorder induces endoplasmic reticulum stress and further promotes neuronal apoptosis.