动物造模,肾缺血再灌注 z-bio 2021-10-15 699

　　OBJECTIVE: To explore the effects of repeated hypoxic preconditioning (RHP) on liver injury caused by renal ischemia-reperfusion injury (IRI) in rats and to preliminarily explore its mechanism.

　　Methods: 120 male SD rats were randomly divided into 4 groups (n=30): hypoxic preconditioning surgery group (RHP group), hypoxia preconditioning sham surgery group (RHPS group), and normal pressure surgery group (IRI group) And normal pressure sham operation group (group S). Rats were pretreated in a hypobaric oxygen chamber for 5 days to establish a renal IRI model. The right kidney was removed in the RHP group and the IRI group, and the left kidney renal hilum was clamped for 45 minutes. The renal ischemia reperfusion model was established, while the RHPS group and S group were released. Only the right kidney was removed, and the left kidney was not subjected to ischemia-reperfusion treatment. Detect serum alanine aminotransferase (ALT), IL-17A, TNF-α concentrations at 2, 8, and 24 h after reperfusion, and detect liver homogenate superoxide dismutase (SOD) and nitric oxide (NO) with a microplate reader Western blot was used to detect the levels of p-PI3K and p-AKT in the liver, and the liver structure changes were observed by pathological tissue morphology.

　　Results: Compared with the IRI group, the histopathological examination of rats in the RHP group at 2, 8, and 24 h after reperfusion showed that the injury was reduced, the serum ALT concentration was reduced, and the TNF-α level was reduced at 24 h after reperfusion (P<0.05) ) NO content in liver tissue increased (P<0.05), SOD content increased 8 hours after reperfusion (P<0.05); compared with S group, serum IL-17A concentration in IRI group and RHP group increased significantly ( P<0.05), but="" the="" difference="" between="" two="" groups="" was="" not="" statistically="" significant="" p="">0.05); the expressions of P-PI3K and P-AKT in the RHP group were higher than those in the IRI group (P<0.05), and the difference was particularly significant at 8 h after reperfusion Significant (P<0.05).

　　Conclusion: RHP has a protective effect on liver damage caused by IRI in rat kidneys, but it is not achieved by inhibiting IL-17A.