动物造模,哮喘小鼠 z-bio 2021-10-15 300

　　Objective: To discuss the effect of chloroquine on airway hyperresponsiveness of asthmatic mice and its possible mechanism.

　　Method: Balb/c mice were randomly divided into control group, asthma group, chloroquine treatment group, dexamethasone treatment group, chloroquine + dexamethasone treatment group. Except for the control group, mice in all other groups were given OVA sensitization and stimulation. Established a mouse model of asthma, administered intraperitoneal injection before each arousal, and the control group was treated with the same amount of PBS at the same time. Within 24 hours after the last arousal, the mouse lung function meter was used to detect the high airway response of the mouse. Detecting the total number of cells in bronchoalveolar lavage fluid (BALF) and classification counts; making pathological sections of lung tissues, and inspecting pathological changes under light microscope after HE staining; detecting the levels of cytokines IL-6 and PGF2α in bronchoalveolar lavage fluid by ELISA .

　　Results: Chloroquine can significantly reduce the airway hyperresponsiveness of asthmatic mice (4.5±0.4 vs 6.1±0.9 when inhaling 25 mg/ml Ach, P<0.05, and 4.6±0.5 vs 8.2±1.0 when inhaling 50 mg/ml Ach, P <0.001); significantly reduce the number of inflammatory cells in the bronchoalveolar lavage fluid of asthmatic mice (P<0.01); significantly reduce the pathological score of asthmatic mice (P<0.05); it can reduce the level of PGF2α factor in the BALF of asthmatic mice to a certain level; Combination with dexamethasone can significantly reduce the inflammation around the bronchi and bronchiole of asthmatic mice (P<0.05), the inflammation around the blood vessels (P<0.01), and the pathological score of lung tissue (P<0.001), and significantly reduce IL- in the BALF of asthmatic mice. 6 factor degree (P<0.05).

　　Conclusion: Chloroquine can inhibit the airway hyperresponsiveness of asthmatic mice; the combined use of dexamethasone and chloroquine is more effective than the single medication, and may be effective for asthma involving neutrophils.