动物造模,肺缺血再灌注损伤 z-bio 2021-10-15 265

　　Objective: To investigate the effect of emulsified fluorocarbon combined with ligustrazine on lung ischemia-reperfusion injury and its mechanism.

　　Methods: A rat lung ischemia-reperfusion model was established and randomly divided into control group (C group), ligustrazine group (T group), emulsified fluorocarbon group (P group) and emulsified fluorocarbon combined with ligustrazine group (TP group) , 10 animals in each group, were administered via the tail vein 5 minutes before the blood supply was restored, and the animals were sacrificed 3 hours after the blood supply was restored. The lung tissues were taken to determine the malondialdehyde (MDA) and myeloperoxidase (MPO) , Superoxide dismutase (SOD), tumor necrosis factor-α (TNF-α) content, observe the pathological changes of lung tissue and score.

　　Results: The content of MDA and MPO in the lung tissues of the T, P, and TP groups was significantly lower than that of the C group (P<0.05), and the MPO content of the TP group was significantly lower than that of the T and P groups (P<0.05); the T and P groups The SOD content of lung tissue in TP group was significantly higher than that in group C (P<0.05), SOD activity in TP group was significantly higher in group T and P (P<0.05); 4="" there="" was="" no="" significant="" difference="" in="" content="" lung="" tissue="" of="" groups="" p="">0.05); Obvious edema was seen in the lung tissue of the C group, and red blood cells and exudation were seen in the alveolar cavity; there was no obvious edema in the lung tissues of the T group and P group, but a small amount of red blood cells and exudation were seen; Exudation; the pathological score of group C was greater than that of group T, P and TP, and the pathological score of group T and P was greater than that of TP group (P<0.05).

　　Conclusion: Emulsified fluorocarbon and ligustrazine can improve the endogenous scavenging ability of oxygen free radicals, inhibit the accumulation of neutrophils in the lung, and reduce lung ischemia-reperfusion injury. The combination of the two has a better effect.