动物造模,帕金森 z-bio 2021-10-15 399

　　Objective: To observe the preventive and therapeutic effects of Ginkgo biloba extract on 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced Parkinson's disease (PD) mice of different ages .

　　Methods: 60 C57BL/6 mice of 8 weeks and 32 weeks of age were randomly divided into negative group, model group, high, medium, and low dose GBE administration group and positive group. The PD animal model was prepared by intraperitoneal injection of MPTP. , Ginkgo biloba extract is used for intragastric treatment. Climbing, hanging and swimming experiments were used to detect the motor function of mouse limbs, and the activity of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and malondioxide in mouse brain tissue was measured by spectrophotometry. Aldehyde (MDA) content, immunohistochemical method was used to observe the changes in the number of positive cells of substantia nigra tyrosine hydroxylase (TH) and striatal dopamine transporter (DAT) in mice.

　　Results: (1) Compared with the negative control group, the motor function scores, brain GSH-Px activity, substantia nigra TH positive cells and striatal DAT positive cells of the 8-week-old model group were significantly decreased, and the brain tissue The MDA content increased significantly, indicating that the PD model was successfully replicated. Compared with the model group, the motor function scores, SOD and GSH-Px activities, and the average optical density of TH-positive cells in the substantia nigra of the 8-week-old mice in the Ginkgo biloba extract administration group were significantly increased, and the MDA content was significantly decreased. (2) Compared with the negative control group, the 32-week-old model group mice's motor function score, the average optical density of substantia nigra TH-positive cells and striatum DAT-positive cells all significantly decreased, SOD and GSH-Px activities decreased, and brain tissue The increase in MDA content indicates that the model is successfully replicated. Compared with the model group, 32-week-old mice in the Ginkgo biloba extract-administered group had significant increases in motor function scores, SOD activity, substantia nigra TH and DAT positive cells in the striatum, and significantly decreased brain MDA content.

　　Conclusion: Ginkgo biloba extract has a certain preventive effect on PD mice of different ages induced by MPTP.