动物造模,2型糖尿病 z-bio 2021-10-15 667

　　Objective: To explore the possible relationship between two-color coreopsis alcohol extract, intestinal flora and type 2 diabetes (T2DM).

　　Methods: Collect model group (A), two-color coreopsis alcohol extract high-dose group 1.8 g/kg (B), two-color coreopsis alcohol extract medium-dose group 1.2 g/kg (C), and metformin control group 0.2 g /kg (D), blank control group (E) stool samples, 16S rDNA real-time fluorescent quantitative PCR technology was used to detect the levels of Clostridium sphaeroides and Bacteroides polymorpha in stool samples; Pearson analysis method was used to determine the level of target bacteria The fasting blood glucose (FBG) of mice in the group was analyzed for correlation.

　　Result: ①Compared with the normal group, the levels of Clostridium sphaeroides and Bacteroides polymorpha in the model group were increased (P=0.017, P=0.002). ②Compared with the model group in the high-dose two-color coreopsis alcohol extract group, the levels of Clostridium sphaericus and Bacteroides polymorpha decreased, and there was a statistical difference (2 weeks: P=0.027, P=0.006; 4 weeks: P =0.007, P=0.012). ③The level of Clostridium sphaericus and the level of Bacteroides polymorpha are positively correlated with the level of FBG.

　　Conclusion: The alcohol extract of two-color coreopsis has a certain effect on the intestinal flora of type 2 diabetic mice and is related to its blood sugar level.