动物造模,缺血再灌注 z-bio 2021-10-15 727

　　Objective: To study the effect of morroniside on the expression of tissue inhibitor of metalloproteinase (TIMP) in rat cortex after 7 days of ischemia-reperfusion.

　　Methods: Twenty-five male SD rats were prepared with a modified Zealonga suture method to prepare a rat middle cerebral artery occlusion (MCAO) model. After the operation, they were randomly divided into model group, low-dose morroniside group, middle-dose group, High-dose group, 5 in each group. Three hours after modeling, morroniside was intragastrically administered at doses of 30, 90, and 270 mg/kg once a day. Western Blotting and Immunofluorencent staining were used to analyze the effects of morroniside on the expression of TIMP in the affected cortex of rats after 7 days of cerebral ischemia and reperfusion.

　　Results: After 7 days of cerebral ischemia and reperfusion, the expression of TIMP protein in the model group was significantly increased compared with the sham operation group (P<0.01); compared with the model group, the morroniside medium-dose group (90 mg/kg) And the high-dose group (270 mg/kg) TIMP protein expression was significantly increased (P <0.05; P <0.01).

　　Conclusion: Moroside can up-regulate the expression of TIMP in the cerebral cortex, which helps to protect and repair the blood-brain barrier after stroke.