动物造模 z-bio 2022-02-06 245

　　Objective: To discuss the effect of curcumin on the survival rate of rats in the desert dry and hot environment.

　　Method: 40 SPF male SD rats aged 6-8 weeks were selected and randomly divided into control group, low-dose curcumin pre-treatment group, middle-dose curcumin pre-treatment group, high-dose curcumin pre-treatment group, and solvent group. The control group was fed normally; the low-dose group, the medium-dose group, the high-dose group, and the solvent group were given equal-volume intragastric administration for 7 consecutive days. Placed in the artificial experiment cabin in the special environment in the northwest region, set the climate form of the desert dry and hot environment (temperature 41℃, humidity 10%, ultraviolet radiation 40W/m2). Observe the vital signs of the rats in each group and monitor the changes in central body temperature every half an hour, and record the time of death.

　　Result: The five groups of rats were placed in the desert dry and hot environment, 0-30 minutes, the core body temperature of the three doses of curcumin was not significantly different from that of the control group and the solvent group (P>0.05). Starting from 60 min, at 7 time points of 60 min, 90 min, 120 min, 150 min, 180 min, 210 min, and 240 min, the core body temperature of the low-dose group, the middle-dose group, and the high-dose group was lower than that of the control group and the solvent group. , The difference is significant (P<0.05). 240="" from="" p="">0.05). The average survival time of the five groups of rats in the control group, curcumin low, medium and high dose treatment group and solvent group were (222.95±28.23) min, (231.35±40.96) min, (255.00±30.39) min, and (263.80±56.71), respectively. )min, (223.00±27.23)min. The survival time of rats in the middle and high dose curcumin pretreatment group was significantly better than that of the low concentration pretreatment group, control group and solvent group (P<0.05), and the high dose group had a significant effect (P<0.01).

　　Conclusion: Curcumin pretreatment can improve the survival time of rats in the desert dry and hot environment, suggesting that curcumin may play an important role in improving the heat tolerance of rats in the desert dry and hot environment, and curcumin may play an important role in the prevention and treatment of heat stroke in the desert dry and hot environment. It has potential clinical application value.