动物造模,非酒精性脂肪性肝病 z-bio 2021-10-18 291

　　Objective: To study the dynamic changes and significance of oxidative stress and cytokines in the composition of non-alcoholic fatty liver disease (NAFLD) in gerbils.

　　Method: 48 male gerbils were randomly divided into normal group and model group, 24 in each group, fed with normal diet and high-fat diet, and disposed of in each group on the 4th, 8th and 16th weekend of the experiment. 8 gerbils, dynamic detection of liver tissue homogenate MDA content and vitality of SOD, GSH-PX and T-AOC by chemical method, liquid suspension chip technology dynamic detection of serum TNF-α, INF-γ, IL-10 and other cytokines .

　　Results: With the cessation of NAFLD disease, the content of MDA in the liver tissue of gerbils gradually increased, and was significantly higher than that of the normal group (P<0.01); the degree of T-AOC first increased slightly and then decreased, compared with the normal group at 4 and 16 weeks Significant decrease (P<0.05); SOD degree first increased significantly and then decreased significantly, at 4 weeks, it was significantly higher than the normal group (P<0.05), and at 8 weeks and 16 weeks, it was significantly decreased (P<0.05, P<0.01); GSH The degree of -PX showed a gradual decrease trend, which was significantly lower than that of the normal group at 8 weeks and 16 weeks (P<0.05). Serum cytokines TNF-α and IFN-γ gradually increased with NAFLD pause, and the level of IL-10 gradually decreased. There were statistically significant differences from the normal group at 8 weeks and 16 weeks (P<0.05, P<0.01).

　　Conclusion: The oxidative stress-related indicators and inflammatory cytokines of the NAFLD model of Mongolian gerbils induced by high-fat show significant changes with the development of simple fatty liver to steatohepatitis, liver fibrosis and cirrhosis, and participate in the onset and development of NAFLD .