动物造模 z-bio 2021-10-18 442

　　The research group of Professor Yuan Jing from the School of Life Sciences of Xiamen University applied the CRISPR/Cas9 system to efficiently complete different types of modifications of multiple genes in the Plasmodium genome, including gene deletion, gene tagging, and allele exchange.

　　Malaria is an infectious disease caused by the infection of Plasmodium parasites. It is still a serious public health burden on a global scale. Although the Plasmodium genome has been sequenced, the function of most genes in its genome is unknown. The original methods of editing and modifying the Plasmodium genome are extremely inefficient, which greatly limits the reminder of the function of the Plasmodium gene.

　　In this seminar, Professor Yuan Jing’s research group from the School of Life Sciences of Xiamen University introduced a site-specific DNA double-strand break into the Plasmodium yoelii genome based on a new genome modification method CRISPR/Cas9 system. Source recombination and repair can efficiently complete different types of modifications of multiple genes in the Plasmodium genome, including gene deletion, gene tagging, and allele exchange. The development of this system has improved the researchers' ability to modify the Plasmodium genome, thereby facilitating the study of Plasmodium biology.

　　The research paper was published in mBio, a journal of the American Society for Microbiology. Researcher Zhang Cui (Master 2), Xiao Bo (Master 2), Jiang Yuan (Master 1) and Zhao Yihua (Master 1) are the co-first authors of this article.