动物造模,1型糖尿病 z-bio 2021-10-20 247

　　Objective To investigate the function of nitrergic nerve and cholinergic nerve of jejunum smooth muscle in rats with type 1 diabetes in the early stage, as well as the morphological changes of jejunum.

　　Method Male SD rats were randomly divided into a normal control group and a diabetes model group, and a diabetic rat model was established by intraperitoneal injection of streptozocin (STZ) 60 mg/kg. 4 weeks later, the application of field stimulation to induce neurogenic contraction response, and the use of isolated organ muscle tension analysis method to observe the contraction response of the isolated jejunum smooth muscle, as well as the changes in the function of nitrergic nerve and cholinergic nerve; paraffin tissue section for HE Staining and immunohistochemical detection, observe the morphological changes of jejunum tissue.

　　Results Compared with normal rats, STZ-induced diabetic rats had impaired cholinergic nerve function in the intermuscular jejunum, but no obvious changes were seen in the function of nitrergic nerves. The jejunum tissue of diabetic rats was chaotically broken. The extra layer thickens, the goblet cells decrease, and the ratio of the length of the villi to the depth of the intestinal crypts (the ratio of the chorionic glands) decreases.

　　Conclusion In the early type 1 diabetes rat model 4 weeks after STZ permeation, the nerve function and morphology of the jejunum tissue have undergone significant changes, suggesting that the intestine, as a sensitive organ for diabetes, plays an important role in the early diagnosis and prevention of diabetes. Clinical reference significance.