动物造模,实验动物 z-bio 2021-10-20 611

　　Objective Magnetic fluid suspension ventricular assist device (hereinafter referred to as blood pump) is actively shut down in vivo experimental animal research, aiming to observe the reflux of blood after stopping the pump and the blood compatibility and biocompatibility of the blood pump, as well as experimental animals Physiological state, accumulate reliable data for later experiments and clinical applications.

　　Method 1 healthy sheep was selected, and the left side chest was opened to establish a left heart assist model. After 6 weeks of normal operation, the pump was automatically stopped and the anticoagulant warfarin was stopped. Observe the physiological status of the experimental animals, record the blood, biochemical, and coagulation results of the sheep before the operation of the blood pump and 1 to 12 weeks and conduct research. Ultrasonography was performed 16 weeks after the blood pump was shut down.

　　Results The left heart assist model was successfully established. The working condition of the blood pump was good during the operation of the blood pump. The experimental sheep had a good mental state, free movement, good eating, and normal urine and urine 6 weeks before and after the blood pump was shut down. Ultrasound performed 16 weeks after the blood pump was shut down showed that the artificial blood vessel was not blocked and had reverse blood flow.

　　Conclusion This blood pump stopped working, and it has no obvious effect on blood compatibility, physiological state and organ function of experimental sheep. The artificial blood vessel is unobstructed and there is no thrombus, and it has no obvious effect on left heart function. Provide a certain reference basis for the treatment of accidental pump stop in the later clinical application process. This blood pump has good blood compatibility. Follow-up will be left ventricular assisted low-dose anticoagulation therapy and multiple groups of shutdown experiments for continuous improvement.