动物造模 z-bio 2021-10-20 589

　　Objective To establish a new mouse resource that reflects the characteristics of genetic diversity at the population level, and to preliminarily explore its genetic characteristics.

　　Methods (1) Multiple parents used recombination hybridization to establish inbred and outbred mice carrying multiple parental genetic information; (2) Through full exome sequencing, the genetics of the two mouse populations were compared and analyzed Scientific characteristics; (3) Through the analysis of the genome sequence of the offspring and parent strains, draw the chromosome physical map of the genetic diversity mouse resources, and count the genetic contribution of the parents to the offspring mice.

　　Results (1) Genetic diversity inbred mice have the characteristics of genetic stability and consistent phenotype, which are suitable for simple and rapid phenotype-genotype correlation analysis; (2) Compared with inbred lines, genetic diversity is far Breeding mice have better reproductive ability and richer genetic variation. Each outbred mouse is a unique individual, suitable for more fine-grained micro-regulation genetic mechanism research. The chromosomes of these outbred mice showed more mosaic characteristics; (3) The genetic contribution of the original parents to the progeny lines is not evenly divided, and there is a genetic preference.

　　Conclusion Compared with traditional mice with a single genetic background, the genetic diversity of inbred and outbred mouse resources established through multiple parents have their own advantages and complement each other in the study of systemic genetics. They are the genetic mechanism of complex traits. The research provides a powerful tool.