动物造模 z-bio 2021-10-20 533

　　Objective To explore the feasibility and application value of testing materials for SPF experimental mouse barrier facilities.

　　Method Collect the experimental materials brought into the barrier facility, conduct bacterial culture on non-cellular samples, and detect mycoplasma by PCR on cellular samples.

　　Results A total of 14 708 experimental materials were collected in 5 years, including 13 085 non-cellular samples, with an average detection rate of 1.99% for bacteria; 1 623 cases with cell samples, with an average detection rate of 4.81% for mycoplasma. The overall positive detection rate has been declining year by year, and has stabilized at a relatively low level in recent years.

　　Conclusion It is feasible to carry out large-scale continuous inspection and control of experimental materials brought into the barrier facility, which can prompt researchers to strengthen the aseptic treatment of animal experimental materials, and enable managers to determine the degree of harm from experimental materials. It is also an SPF-level experimental mouse microorganism. One of the effective measures for infection prevention and control.