动物模型 z-bo 2020-08-10 504

　　The third lumbar transverse process syndrome is a common disease in human clinical orthopedics. The disease is mainly caused by the posterior lateral branch of the second lumbar vertebral nerve passing through the dorsal side of the transverse process and down into the sacral spinous muscle. The surrounding muscle fibers, ligaments and other tissues attached to the transverse process are caused by aseptic inflammation of the skeletal-muscular system Adhesion and scarring due to injury.

　　(1) Method of replication The experimental rat (or rabbit) was injected with 10% Sumianxin anesthetized intramuscularly at a dose of 25mg/kg body weight, and then a 3cm longitudinal incision was made on the back of the rat (or rabbit) aseptically. The sacral spinal muscles were bluntly stripped on both sides to expose the spinous processes of the third to fourth lumbar vertebrae (L3～L4). Use micro orthopedic instruments to treat the surrounding tissues to the lamina, and carefully protect the tissue structure where the lumbar nerve exits the intervertebral foramen. The posterior branch of the deep fascia of the lumbar spine passes through. Then cut out a gelatin sponge with a size of about 0.5mm×0.5mm, and implant it under the middle layer of the deep fascia of the posterior half of the transverse process of the third lumbar vertebra, leaving the intact myofascial membrane to stop bleeding completely. Both the sacral spinal fascia and skin were sutured with 3 stitches equidistantly with 0-gauge thread, and the incision was repeatedly washed with gentamicin.

　　(2) Characteristics of the model: Animals have obvious mechanical irritation and hypersensitivity in the early postoperative period. Histopathological observation of the third lumbar vertebra joint segment of the animal showed vasodilation, congestion, and nerve fiber edema on the 4th day after operation. The muscle inflammatory reaction was severe, the connective tissue and fascia reaction were light, and there was no foreign body cell reaction around the material. There was still tissue congestion 10 days after the operation, and the inflammatory reaction of muscle and internal connective tissue was similar. The inflammatory cells were denser, the materials had been disintegrated, and most of them were absorbed by the cells. Thirty days after the operation, the material has been completely absorbed, the inflammatory reaction in the muscle is mild, and the damage has been repaired. Cells in the tendon fascia gather, fibroblasts begin to proliferate, collagen reconstruction begins, and original scar formation can be seen. The plasma test results showed that the levels of prostacyclin (related to the degree of congestion) and serotonin (pain can be caused by inflammation at low concentrations) in the early postoperative period increased significantly.

　　(3) Comparative Medicine The transverse process of the third lumbar vertebra has a special position and function in the lumbar spine, and the tensile stress on its end is greater than that of other lumbar transverse processes. When the lumbar spine is under excessive force or is in a bad working position for a long time, the third lumbar spine is the longest, so the force it receives as a lever is the largest. All the ligaments, muscles, fascias and tendons attached to it also bear the greatest pressure and are more prone to damage, causing acute and chronic damage to the end tissues of the transverse process of the third lumbar vertebra, especially the involvement of blood vessels that affects the supply of local tissues, causing or Exacerbate the pathological changes of the end tissue of the transverse process of the third lumbar vertebra.