动物造模 z-bio 2021-10-25 345

　　Objective: To study the growth and development characteristics and neurobehavioral characteristics of young rhesus monkeys 12 months after birth, and to establish background reference data for growth and development parameters.

　　Method: Select 18 young rhesus monkeys (11 males and 7 females) born under single cage conditions, with clear genetic background and clear days of birth. Weight and height are measured monthly from day 1 to day 360 after birth Measurement of growth and development indicators such as head circumference, bust circumference, forelimb length, hind limb length, crown buttocks length, tail length and anogenital distance, hematology, blood biochemistry, and lymphocyte subsets at 28, 175, and 360 days after birth Analyzing and testing, a finger labyrinth test was performed on the 208th day after birth.

　　Results: The body weight increased linearly, and there was no significant difference between the sexes (P>0.05); in the morphological development indexes, the distance between males and females was significantly greater than that of the anogenitals (P<0.01). there="" were="" no="" significant="" differences="" between="" the="" sexes="" at="" each="" test="" point.="" sex="" p="">0.05); there was no significant difference between the sexes in the indicators of hematology (P>0.05), and the RBC and HGB on the 175th and 360th days after birth were significantly higher than that on the 28th day (P<0.05); it was compared with that on the 28th day after birth. Compared with 28 days, the levels of TP and BUN in blood biochemistry were significantly increased at the 175th and 360th days after birth (P<0.01). although="" alp="" showed="" a="" the="" difference="" was="" not="" significant="" p="">0.05). Compared with the 28th day, the CD4+ and CD4+/CD8+ ratios in the lymphocyte subsets were significantly reduced on the 175th and 360th days after birth (P<0.01), while the CD8+ ratio was significantly increased (P<0.01); in the finger labyrinth test The number of tests required to complete task 2 in the learning period of the young monkeys was significantly more than that of other tasks, and the females were significantly less than the males (P<0.05), and="" there="" was="" no="" significant="" difference="" between="" the="" genders="" in="" remaining="" tasks="" p="">0.05); the memory period was random for 2 consecutive days The accuracy of testing female young monkeys was significantly higher than that of male young monkeys (P<0.05).

　　Conclusion: The body weight and morphological development indicators increase linearly with the number of days of birth. The indicators of hematology, blood biochemistry, and lymphocyte subsets include RBC, HGB, LYMPH, TP, BUN, ALP, CD4+, CD8+ and CD4+/CD8+. The changes related to the growth and development of the baby monkey’s organ system should be paid attention to in the drug evaluation. The finger labyrinth test suggests that the female baby monkeys are better than male baby monkeys in reversing learning ability and long-term memory. In this study, the background data and neurobehavioral characteristics of growth and development parameters of young rhesus monkeys 12 months after birth were established, which provided reference and data support for the evaluation of rhesus monkey reproductive and developmental toxicity.