动物造模 z-bio 2021-10-25 204

　　Objective: To establish a SNP typing method for frozen embryos and sperm in mice, which can be used for rapid genetic identification of frozen embryos and sperm.

　　Methods: Using frozen mouse embryos and sperm provided by Shanghai Laboratory Animal Center of Chinese Academy of Sciences (Shanghai Branch of National Rodent Experimental Animal Seed Center) as samples, whole genome amplification technology and PCR-LDR typing technology were used to establish mouse frozen SNP Genetic identification method.

　　Results: Whole genome amplification technology can greatly increase the total amount of DNA in frozen embryo samples; PCR-LDR typing method is suitable for the typing of 45 SNPs in the mouse genome; the typing is determined to be C57BL/6, BALB/c, FVB There are 10 inbred lines of embryos and sperms such as /NJ, and the SNP locus information is consistent with the sequencing results; the number of frozen mouse embryos is directly proportional to the number of SNPs detected. When the number of embryos reaches 12 or more, the SNP detection rate is 100% .

　　Conclusion: To achieve rapid SNP genotyping and genetic quality identification of frozen embryos and sperm of inbred mice.