动物造模,树鼩乳腺肿瘤 z-bio 2021-10-25 172

　　Objective To establish a tree shrew breast tumor model.

　　Method Using DMBA combined with synthetic progesterone MPA method, 45 female tree shrews were selected and randomly divided into 3 groups. (1) DMBA group: DMBA (20 mg/time) gavage treatment for 3 consecutive times, once a week; (2) DMBA+MPA group: once every 3 weeks, after 3 consecutive DMBA gavage treatments, MPA sustained-release tablets (150 mg/tablet, 90 d sustained-release) were implanted subcutaneously on the left side of the back of the tree shrew for the first time, 3 months apart, and MPA tablets were implanted for the second time; (3) Normal control group: peanut oil Gavage was performed once every 3 weeks for 3 consecutive times. After the experimental treatment, the tumor occurrence was observed regularly every week for a total of 45 weeks. HE staining was used to identify the pathological types of induced tumors.

　　Results DMBA alone can induce tree shrews to specifically produce breast tumors, with an induction rate of 12%; combined with MPA subcutaneous implantation can increase the incidence of DMBA-induced breast tumors to 50%; while the control group did not observe the occurrence of breast tumors. The induced tumors were mainly intraductal papilloma with low malignancy. Only one case was invasive ductal carcinoma with high malignancy.

　　Conclusion The induced tree shrew intraductal papilloma and invasive ductal carcinoma are the common tumor pathological types in humans. The induced tumor morphology is similar to that of spontaneous tumors.