动物造模 z-bio 2021-10-25 267

　　Purpose: To observe the changes of inflammatory response after space-mutated E. coli infected tail-hanging mice with simulated weightlessness.

　　Methods: Forty C57BL/6 mice were randomly divided into control, control infected, tail hanging and tail hanging bacterial groups. ELISA and RT-qPCR methods were used to detect the inflammatory factor TNF-α in mouse plasma and intestinal tissues. , IL-1β, IL-6 content and mRNA expression, HE staining to observe the changes of small intestine tissue morphology.

　　Results: The results of plasma inflammatory factor ELISA and intestinal tissue inflammatory factor PCR showed that compared with the control group, the expression of inflammatory factors TNF-α, IL-1β and IL-6 in the plasma and intestinal tissue of the experimental group increased. , And the tail hanging bacteria group was the most significant (P<0.01 or P <0.001); the HE staining results of the small intestine tissue showed that the small intestinal mucosa of the experimental group had varying degrees of damage, and the tail hanging bacteria group was the most serious.

　　Conclusion: Space mutagenic Escherichia coli infection can significantly increase the expression of inflammatory factors in plasma and intestinal tissues in tail-hanging mice, leading to more serious damage to the intestinal mucosal barrier, suggesting that tail-hanging simulated weightlessness is infected with space mutagenesis of Escherichia coli Can cause the body's inflammatory response to increase.