动物造模,脓毒症 z-bio 2021-10-25 445

　　OBJECTIVE: To study the effect of pre-infusion of necrotic cells on the balance of Treg/Th17 cells in a mouse model of sepsis.

　　Methods: 34 C57BL/6 mice were randomly divided into 4 groups: normal control group (PBS group, n=5), sham operation control group (Sham group, n=5), sepsis group (CLP group, n= 12) and necrotic cell treatment group (Nec group, n=12). The PBS group was a normal mouse, and 200 μl PBS was injected into the abdominal cavity once. The Sham group was surgically separated from the terminal cecum without ligation and perforation; the CLP group was used cecal ligation and perforation (CLP) to establish a sepsis model; the Nec group was small The mice were injected with 2×107 necrotic cells in the intraperitoneal cavity in advance, and the cecum was ligated and perforated on the 5th day. All mice were sacrificed 2 weeks after preparation of CLP model, and the ratio of CD4+Foxp3+Treg cells and CD4+IL17A+Th17 cells in peripheral blood, spleen and thymus was detected by flow cytometry.

　　Results: The Th17 ratio and Treg/Th17 ratio in the spleen of the CLP group were significantly higher than those of the PBS group and the sham group (P<0.01); the ratio of Treg in the thymus of the CLP group was significantly lower than that of the Sham group (P<0.01); The injection of necrotic cells can reverse the imbalance of Th17 ratio, Treg ratio and Treg/Th17 ratio in mice after CLP surgery (P<0.05).

　　Conclusion: Pre-infusion of necrotic cells can reverse the imbalance of Treg/Th17 cell subsets in septic mice.