动物造模,内毒素血症 z-bio 2021-10-25 453

　　OBJECTIVE: To analyze the changes of miRNA expression profile in myocardial tissue of rats with endotoxin, also known as lipopolysaccharide (LPS)emia, and to explore the role of miRNA in endotoxemia myocardial injury.

　　Method: 20 male SD rats were randomly divided into control group (n=10) and LPS group (n=10). The LPS group was intraperitoneally injected with LPS 10 mg/kg, and the control group was intraperitoneally injected with the same amount of normal saline. Rats were sacrificed by cervical dislocation after 24 hours, and myocardial tissue was taken. Real-time PCR was used to detect the expression levels of TLR4, TNF-α, and IL-1β, and the ultrastructural changes were observed by transmission electron microscopy. The miRNA chip screens the differentially expressed miRNAs in myocardial tissues, and Real-time PCR verifies the actual expression levels of candidate miRNAs.

　　Results: The expressions of TLR4, TNF-α, IL-1β in myocardial tissue of rats in the LPS group were significantly increased, cytoplasmic vacuoles occurred in myocardial cells, mitochondrial edema, structural destruction and other ultrastructural changes. Chip technology and Real-time PCR confirmed that miR-194-3p, miR-344a-3p, miR-465-3p, miR-501-5p, miR-3596c, miR-185-3p, miR in the myocardial tissue of rats in the LPS group -877 was significantly up-regulated, miR-208b-3p, miR-547-3p, miR-141-3p, miR-28-5p, miR-3585-5p were significantly down-regulated.

　　Conclusion: These differentially expressed miRNAs found in the myocardial tissue of endotoxemia rats may be related to the occurrence of myocardial damage.