动物造模 z-bio 2021-11-02 303

　　Objective To compare the behavioral differences of C57BL/6J, BALB/c, and ICR strains in the learning and memory ability of mice.

　　Methods According to strains, 10-week male C57BL/6J, BALB/c, and ICR mice were divided into three groups, each with 10 mice, and the open field experiment, Barnes maze experiment, Y maze experiment, and conditioned fear experiment were performed, and the three strains were compared. Mice learning and memory ability.

　　Results In the open field experiment, the total moving distance, large moving distance, speed, central area moving distance percentage, central area stay time in the C57BL/6J group was less than that of the ICR group and the BALB/c group, and the difference was statistically significant (P<0.01, P <0.05); C57BL/6J group has more small movement distances than ICR group and BALB/c group, and the difference is statistically significant (P<0.01, P<0.01); Barnes maze test stage, C57BL/6J group and ICR group The staying time in the target area was longer than that of the BALB/c group, and the difference was statistically significant (P<0.01, P<0.05). There were no statistically significant differences in the spontaneous conversion rate, staying time and number of arms in the three groups of mice in the Y maze experiment. In the conditioned fear experiment, there was no statistically significant difference in the percentage of freezing time between the three groups of mice.

　　Conclusion Among the three strains, C57BL/6J mice have the lowest spontaneous activity and the highest level of anxiety in the open field experiment. The spatial reference memory is better than the ICR group and BALB/c mice. There is no significant difference in the spatial working memory and spatial recognition memory of the three strains.