动物造模,荧光RPA检测 z-bio 2021-11-02 191

　　Objective In order to improve the surveillance and epidemiological investigation of the inbound and outbound wild and experimental primate monkey T lymphotropic virus type 1 (Simian T-cell lymphotropic virus type 1, STLV-1) infection at the port, this study established Recombinase polymerase amplification (RPA) and fluorescent RPA methods to detect STLV-1.

　　Methods Use software to analyze the gag polyprotein (gag polyprotein) gene sequence of STLV-1 isolated from different countries and regions, design RPA primers and probes, establish RPA and fluorescent RPA methods to detect STLV-1, and be sensitive to the specificity and sensitivity of the method Performance and stability are verified.

　　Results By comparing other specific pathogens in monkeys with STLV-1, it was confirmed that the established detection method had good specificity; through sensitivity tests, it was confirmed that the established detection limits of RPA and fluorescent RPA methods were consistent with PCR; through the comparison of 30 STLV- 1 The detection of positive and negative nucleic acid samples confirms that the established RPA and fluorescent RPA methods have the same stability and reliability as the PCR method.

　　Conclusion The RPA and fluorescent RPA methods established in this study have good specificity, sensitivity and reliability for the detection of STLV-1, and can be applied to STLV-1 surveillance and epidemiological investigations.