动物造模 z-bio 2021-11-02 348

　　Objective To observe the incidence of spontaneous lesions in SD rats of different ages and genders in different environments, and to provide background information for the pathological evaluation of drug safety studies.

　　Methods The blank control SD rats in the safety evaluation test of our center were used as female piles. They were reared in the barrier system and ordinary environment. They were sacrificed and dissected at 19, 23, 32, and 36 weeks of age, and pathological sections were routinely made. , Observe the pathological changes of major tissues and organs under an optical microscope, and count the types and incidence of spontaneous animal diseases.

　　Results Compared with the barrier system, the incidence of spontaneous lesions in the respiratory system of rats in ordinary environments was significantly increased; in addition, the incidence of spontaneous lesions in SD rats increased with age in the barrier environment and under the conditions of ordinary environment feeding. Trend; At the same time, a small number of lesions show obvious gender differences, of which progressive cardiomyopathy and renal mineral deposits are the most obvious. Histopathological examination showed progressive cardiomyopathy, liver focal inflammatory cell infiltration, hepatocyte vacuolar degeneration, spontaneous variant hepatocyte foci, lung focal inflammatory cell infiltration, foam cell accumulation in the alveoli, renal mineralization, renal interstitium Inflammatory cell infiltration, pancreatic atrophy, tracheitis cell infiltration, prostatic interstitial inflammation, testicular seminiferous tubule atrophy, seminiferous tubule atrophy, shedding of seminiferous epithelial cells, endometrial inflammation and vaginal inflammation are the main spontaneous lesions.

　　Conclusion The types and incidence of various spontaneous lesions in SPF SD rats reported in this article enriched the background data of experimental animals and can provide references for relevant technicians.