动物造模,深Ⅱ度烫伤 z-bio 2021-11-02 635

　　Objective To establish a pig model of deep second degree scald and observe the histopathological changes of skin repair at different time points.

　　Method Prepare the deep second-degree burn wounds of the skin of miniature pigs by using a constant temperature scald instrument. Observe the macroscopic morphological changes of skin wound healing on the 1, 3, 7, 4, 21, and 28 days and measure the wound healing rate. According to the law of skin tissue healing and the operability of the experiment, select the 1. Sampling on 14 and 28 days, using HE, Massom staining and immunohistochemical CD31 staining to observe the dynamic pathological changes of skin repair at different time points.

　　Results By measuring the wound healing rate, compared with female pigs, the healing rate of the scalded wound of the venerative pig is faster. The skin tissue after scald has been confirmed by different staining methods that the damage has accumulated to the deep layer of the dermis, reaching the standard of deep second degree scald. On the 14th and 28th days of the subsequent selection, the skin tissues were repaired to varying degrees after injury. After 28 days of deep second-degree scald, the skin of the mini-pig has basically healed, but the skin tissue is not yet fully mature. Except for the relatively mature layers of the epidermis, the dermal layer has not yet fully formed the scar tissue.

　　Conclusion Compared with female pigs, male pigs have a faster healing rate for scalded wounds.