动物造模,湿热泄泻模型 z-bio 2022-12-29 376

　　OBJECTIVE: To apply UPLC-Q/TOF-MS/MS urine metabolomics method based on ultra performance liquid chromatography-tandem quadrupole time-of-flight mass spectrometry (UPLC-Q/TOF-MS/MS) to screen potential metabolic markers of damp-heat diarrhea, and to explore rats The pathogenesis of damp-heat diarrhea.

　　Methods: High-sugar and high-fat (HF) + high temperature and high humidity (HH) + intraperitoneal injection of enterotoxigenic Escherichia coli (EC) were used to establish a rat model of damp-heat diarrhea. High humidity, poisoning) urine samples, using urine metabolomics technology to analyze the samples, multivariate statistical analysis methods to analyze metabolic data.

　　Results: L-glutamyl-L-cysteine, L-formyl kynurenine, 5-hydroxy-N-formyl kynurenine, riboflavin-5-phosphate, linoleic acid, L-tryptophan were screened out 7 potential metabolic markers such as acid and melatonin.

　　Conclusion: Damp-heat diarrhea involves tryptophan metabolism, riboflavin metabolism and linoleic acid metabolism and other metabolic pathways, indicating that energy, amino acid and lipid metabolism disorders occur in rats. The pathogenesis provides a reference basis.