动物造模,狂犬病毒 z-bio 2021-11-10 954

　　Objective: To establish a mouse anti-rabies virus IgG antibody ELISA detection method for the detection and analysis of rabies mouse models.

　　Method: Determine the best dilution of the sample and the best concentration of the secondary antibody through orthogonal experiments; analyze the specificity, sensitivity, and stability of the method; use it with commercial kits for mouse samples To determine the compliance rate of the ELISA method.

　　Results: The best dilution of the sample of this ELISA method is 1:100, the best concentration of the secondary antibody is 40ng/mL, and the positive cut-off value is 0.121. 129μg/mL; the coefficient of variation of three replicates of the sample is less than 10%. The coincidence rate with the commercial kit is 100%.

　　Conclusion: The ELISA detection method for mouse anti-rabies virus IgG antibody has been successfully established, which can be applied to the analysis of rabies mouse models and the evaluation of vaccine titer.