动物造模 z-bio 2021-11-10 301

　　Purpose: The experiment aims to study the addition of L-arginine to corn agar medium, and to explore the effects of L-arginine on the pupation time, emergence time, emergence rate and male-male ratio of fruit flies.

　　Method: The experiment is divided into 2 groups, namely the control group and the experimental group. The control group is the basal medium. The experimental group adds 1% L-arginine to the basal medium. 7 pairs of fruit flies with 12 replicates in each group. After 72 hours of inoculation, the parents were removed, and the pupation time, emergence time, emergence number and number of males and females in each group were counted.

　　Results: 1% L-arginine was added to the medium to increase the pupation number and emergence number of white eyes (w1118), residual wings (CyO) and wild-type Drosophila melanogaster by 313.64% and 303.53% respectively compared with the control group. P<0.01), 157.09%､182.76%(P<0.01) and 233.33%､239.03%(P<0.01), the pupation time of white eyes and wild-type fruit flies after adding 1% L-arginine was longer than that of the control group 2d in advance.

　　Conclusion: Under the conditions of this experiment, 1% L-arginine can improve the reproductive capacity of laboratory fruit flies and can be used for the propagation of laboratory fruit flies.