动物造模,自发性糖尿病 z-bio 2021-11-10 763

　　Purpose: Diagnose spontaneous diabetic cynomolgus monkeys and analyze the changes in bone microstructure in order to establish an animal model of spontaneous diabetes with osteoporosis.

　　Methods: Four spontaneously diabetic cynomolgus monkeys aged 13-20 years were selected, and animals of the same age were used as controls; fasting blood glucose and glucose tolerance were detected by rapid blood glucose meter, and related biochemical indicators in serum were detected by biochemical meter. Diabetic cynomolgus monkeys were diagnosed; the bone structure of the lower femur and upper tibia was measured by Micro-CT, and the changes in bone microstructure of spontaneous diabetic cynomolgus monkeys were analyzed.

　　Results: The fasting blood glucose of the cynomolgus monkeys in the spontaneous diabetic group was greater than 8.0mmol/L, the blood glucose was greater than 10.0mmol/L at 120 min of the glucose tolerance test, and TG and LDL-C were significantly increased compared with the control group, and HDL-C and Crea were significantly higher. Lower than the control group, there is no significant difference in TC and BUN, which can be diagnosed as spontaneously diabetic animals; Micro-CT results show that spontaneously diabetic cynomolgus monkey bone microstructure destruction is obvious, showing osteoporosis, and the lower femur and upper tibia are cancellous The trabecular bone area, trabecular bone volume and bone mineral content were significantly lower than those of the control group, but there was no significant difference between the lower femur and upper tibia cortical bone.

　　Conclusion: Spontaneous diabetic cynomolgus monkeys have similar clinical characteristics to human diabetic patients, and can be used as an ideal animal model for diabetes and osteoporosis.