动物造模,基因敲除 z-bio 2021-11-12 373

　　Objective: To observe the various parameters of the leptin receptor gene knockout rat model with obesity, metabolic syndrome and pre-diabetes characteristics established by CRISPR/Cas9 technology, and to provide a more ideal model for the molecular mechanism research of metabolic diseases and the development of new drugs Animal model.

　　Methods: 1) Regularly determine the body weight, food intake, random blood glucose and fasting blood glucose of Lepr-/- rats; glucose tolerance test and insulin tolerance test; serum lipid parameters; 2) observe 14-week and 18-week Lepr-/- rats Pathological changes of the main organs; 3) Observe the lipid deposits in the organs.

　　Results: Lepr-/- rats showed obvious obesity, high food intake, impaired glucose tolerance, insulin resistance and dyslipidemia; pancreatic islet hyperplasia and cardiac muscle cell hypertrophy at 14 weeks and 18 weeks, fatty liver, obesity-related nephropathy; liver Cells, renal tubular epithelial cells and lipid deposition in skeletal muscle fibers.

　　Conclusion: Lepr-/- rat is an ideal animal model for the study of the etiology of metabolic diseases and the development of new drugs.