动物造模 z-bio 2021-11-12 217

　　Objective: To study the effect of sodium fluoride on the pathology of rat bone, brain, liver and kidney tissues.

　　Method: Select 20 healthy weaned SD rats, half male and half male, and randomly divided into control group and fluorosis group. Rats in fluorosis group freely drink tap water containing sodium fluoride (fluoride ion concentration is 100mg/L), regularly Weigh, measure body length and tail length, and observe dental fluorosis and symptoms of poisoning. After 12 weeks of feeding, test the fluoride content in femur, liver, kidney and brain tissue of each group of rats, and determine serum, liver, kidney and brain tissues. Aldehyde (MDA) content, observe the pathological structural changes of each tissue under light microscope and electron microscope.

　　Results: The food intake, water intake, body weight, body length, and tail length of the rats in the fluorosis group were slightly lower than those in the control group, but the difference was not statistically significant; the rats in the control group did not have dental fluorosis, and the rats in the fluorosis group had fluorosis The dental incidence rate was 100%; compared with the control group, the fluorine content in the femur, liver, kidney and brain tissue of the fluorosis group was significantly increased (P<0.01); the serum, liver, kidney and brain of the fluorosis group The content of MDA in tissues was higher than that in the control group (P<0.01 or P<0.05). Light microscopy results showed that the trabecula of rats in the fluorosis group had uneven thickness and increased spacing; some liver cells had obvious edema, and the arrangement of hepatocyte cords was disordered ; Renal cortex proximal convoluted tubule epithelial cells swelled and the lumen enlarged; hippocampus nerve cells swelled and vacuolated. Under electron microscope, rat osteoblasts, hepatocytes, renal proximal convoluted tubule epithelial cells and brain neurons in the fluorosis group All of the ultrastructures present the typical characteristics of apoptosis, which are manifested by pyknosis of the nucleus, concentrated chromatin, swelling of mitochondria, and vacuolation. Conclusion Sodium fluoride can cause experimental rat bone, liver, kidney and brain Pathological tissue damage, leading to cell apoptosis.