动物造模 z-bio 2021-11-12 267

　　Objective: To screen the pathogenicity-related genes of enterovirus 71 through genetic diversity mouse resources, to understand the pathogenic mechanism of the virus, and to provide a basis for clinical diagnosis, treatment and prognosis.

　　Methods: 107copies EV71 virus was infected with 4-6 weeks old genetically diverse mouse strains through the abdominal cavity, blood and hind limb skeletal muscle samples were collected, real-time fluorescent quantitative PCR technology was used to analyze the viral load, and the genetic database of The GeneMine system was used to analyze the infection data , Screening and obtaining EV71 pathogenicity-related genes.

　　Result: 53 genes related to the pathogenicity of EV71 were screened through genetic diversity mouse infection data.

　　Conclusion: Genetically diverse mice have great advantages in simulating population genetic diversity and studying complex traits and diseases. They are ideal new animal resources for screening disease-related genes.