动物造模 z-bio 2022-02-01 437

　　Purpose: Apply high-throughput sequencing technology to analyze the structure and composition of the fecal flora of common cotton-eared marmoset monkeys, and lay the foundation for the further development and utilization of new experimental animals.

　　Method: Collect 4 adult male common cotton-eared marmoset feces, use bacterial 16SrRNA universal primers to amplify the V3~V4 region, and use the IlluminaMiSeq sequencing platform to study the common cotton-eared marmoset fecal microorganisms.

　　Results: A total of 315,511 effective sequences and 596 OTUs were obtained by sequencing. A total of 9 phyla, 14 classes, 26 orders, 50 families, 82 genera and 64 species were identified in the bacteria in the feces of common cotton-eared marmosets. 226 OTU. Among them, 1) the dominant phyla are Bacteroidetes and Firmicutes, with an average content of 54.52% and 25.39% respectively. 2) The most abundant classes are Bacteroidia and Firmicutes. Anaerobic bacteria (Negativicutes), with an average content of 54.5% and 17%. 3) Lactobacillales and Bacteroidales have the highest abundance, 50.01% and 20.52%. 4) Prevotella Family (Prevotellaceae) and Bifidobacteriaceae (Bifidobacteriaceae) account for the highest abundance, with an average content of 43.14% and 11.33% respectively. 5) Bifidobacterium and Bacteroides have higher abundance, The average content is 11.33% 11.12% respectively. 6) Bifidobacteria of the beneficial bacteria group are relatively abundant, but they are all contained in the tested samples. 7) The top 10 most abundant species are classified into 7 families. 5 classes. These 10 species accounted for 33.16%, and the other 53 species accounted for only 0.74%. The other species have not been identified, and their relative abundance is relatively high. Further research is needed. 8) PICRUSt function prediction analysis: amino acid Metabolic functions such as transport and protein translation and folding have high abundance of genetic information processing functions.

　　Conclusion: Using high-throughput sequencing technology, the fecal flora of common cotton-eared marmosets was comprehensively detected. The composition of common cotton-eared marmoset fecal bacteria is rich in diversity, and many of them have not been classified and identified and have relatively relatively abundance. High bacteria need further research.