动物造模 z-bio 2021-11-12 254

　　Objective: To explore the effect of myocardial tissue-specific Isca1 knock-out rats on magnetic resonance and pathology analysis.

　　Methods: Breeding myocardial tissue-specific Isca1 knockout rats, PCR technology to identify the rat genotype and gene knockout efficiency, and MRI images were performed on the 0.5-day and 2.5-day new-born myocardial tissue-specific Isca1 knockout rats. H&E staining and transmission electron microscopy analysis were performed on the myocardial tissue of newly born 2.5-day myocardial tissue-specific Isca1 knockout rats.

　　Results: The knockout efficiency of myocardial tissue-specific Isca1 knockout rats was greater than 78%; compared with wild-type, the heart of myocardial tissue-specific Isca1 knockout rats did not show significant expansion; 2.5d myocardial tissue-specific Isca1 knockout The right ventricle of the rat heart shows a tendency to dilate; 2.5d myocardial tissue-specific Isca1 knockout rats have unevenly arranged muscle fibers, appear disordered, no hierarchy or polar orientation, some myocardial fibers have lytic breaks, and sarcomere and Z-line are blurred, appearing Myometrium is damaged, mitochondrial cristae is broken, and the swelling is obvious.

　　Conclusion: Myocardial tissue specific Isca1 knockout caused abnormal heart structure in neonatal rats.