动物造模 z-bio 2021-11-12 505

　　Objective: To determine the type and distribution of influenza virus receptors in different tissues and organs of dogs, and to verify the susceptibility of dogs to H3N2 subtype canine influenza virus.

　　Method: Collect different tissues and organs of healthy English Springer Spaniel, and use lectin immunofluorescence tissue staining method to detect human influenza virus sialic acid receptor sialic acid α2,6-galactose (SAα2,6Gal) and avian type in the tissue Types and distribution of influenza virus sialic acid receptor sialic acid α2,3-galactose (SAα2, 3Gal); artificially infected English Springer Spaniel with H3N2 subtype canine influenza virus, collected different tissues and organs, and performed immunohistochemical staining Analysis of the difference in the tissue distribution of the virus.

　　Results: There are two types of receptors SAα2, 6Gal and SAα2, 3Gal in a variety of tissues and organs, and there is a single receptor in a few tissues and organs; the wide distribution of SAα-2, 6Gal receptors in the same organ The expression level is higher than that of SAα-2, 3Gal receptors. After artificial infection, the overall trend of virus distribution and receptors is basically the same, and there are only differences in individual organs.

　　Conclusion: Avian and human influenza virus receptors are widely distributed in various tissues and organs of dogs, and dogs have the molecular basis for simultaneous infection of avian and human influenza viruses.