动物造模 z-bio 2023-04-21 574

　　Objective: To use PKH26 and Hoechst 33258 to track the location of Uncv hairless mouse iRhom2 and its mutant protein in Vero cells.

　　Method: Stain the cell membrane with PKH26, while staining the nucleus with Hoechst 33258 dye, and observe under a laser confocal microscope.

　　Result: Observing the target protein by laser confocal fluorescence microscope, it is found that wild-type iRhom2 is distributed in the cytoplasm of the cell, and iRhom2mut exists in both the cytoplasm and the nucleus.

　　Conclusion: The difference in subcellular location suggests that the N-terminal deletion mutation of iRhom2 may affect its intracellular location.