动物造模 z-bio 2021-11-25 244

　　Purpose: To study the polymorphism of SLA class Ⅱ genes in experimental SPF large white pigs and Landrace pigs.

　　Methods: Collect 15 SPF large white pigs and 22 Landrace pigs with anticoagulant blood, separate peripheral blood lymphocytes, extract total RNA, and RT-PCR to amplify DQB1, DRB1 and DQA genes after reverse transcription and sequence them. SLA class Ⅱ allele sequence polymorphism.

　　Results: A total of 25 alleles were obtained for 3 genes, including 8 DQB1, 10 DRB1 and 7 DQA, all of which were officially named by the ISAG SLA Nomenclature Committee. Among them, 3 alleles were submitted for the first time with complete sequences and named DQB1 *02: 12 (KU754590), DQB1*02: 03 (KU754591) and DRB1*06: 07 (KU754601), 3 DQA alleles are newly discovered alleles. Among the 15 amino acids of SPF large white pigs and Landrace pigs DQB1 alleles binding to foreign antigens, a total of 5 amino acids are highly conserved; out of the 16 foreign antigen recognition sites of DRB1 alleles, only 1 The sites are highly conserved; among the 19 antigen-binding sites of DQA alleles, 11 are highly conserved. The molecular phylogenetic tree of the amino acid sequence of the SLA class Ⅱ genes showed that the three genes were clustered into two major classes, which are closely related to foreign Yucatan miniature pigs, but do not show significant genetic distance from other pig breeds.

　　Conclusion: Successfully identified 25 SLA class Ⅱ alleles of large white pigs and Landrace pigs, and found that they have relatively abundant polymorphisms. The obtained SLA class Ⅱ alleles are also widely distributed and diverse in other breeds of pigs. The results of this research play an important role in the development of large white pigs and Landrace pigs into classic experimental animal models.